ExFLP-21 Determination of Extraneous Material in White Rice
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18A00066304A40AD94C59CF9741D74A6 |
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文件大小(MB)： |
0.08 |
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页数： |
6 |
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日期： |
2012-3-3 |
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Government of Canada Gouvernement du Canada,Laboratory Procedure ExFLP-21,April 1997,HEALTH PRODUCTS AND FOOD BRANCH,OTTAWA,DETERMINATION OF EXTRANEOUS MATERIAL IN WHITE RICE,Research Division, Bureau of Microbial Hazards,Food Directorate, Postal Locator: 2204A2,Health Canada, Ottawa, Ontario, K1A 0L2,1. APPLICATION,This method is applicable to the sampling and examination of white rice for extraneous material, such as insect,infestation, and rodent hairs, to determine compliance with Sections 4, 5 and 7 of the Food and Drugs Act.,This method replaces ExFLP-21 dated September 1987.,2. DEFINITION OF TERMS,A lot is defined as that amount (volume, weight, etc.) of the food which is produced, stored and/or shipped,under conditions as nearly uniform as possible, preferably designated by a common container code or marking,and, in any event, consisting of not more than one variety, grade or type of product from a single identifiable,source.,3. COLLECTION OF SAMPLES,3.1 Scrutinize the entire lot for live infestation. If live infestation is found, do not sample until after,fumigation or other effective treatment.,3.2 Obtain three sample units selected at random from the lot of at least 100 g each using appropriate,sampling equipment and containers. Three sample units constitute a sample.,3.3 Each sample unit must be kept separate and labelled 1, 2 and 3. Complete information respecting the,lot size, weight of individual containers, country of origin, exporter, importer, or domestic manufacturer,and product and lot identification should be recorded and should accompany the sample.,4. MATERIALS AND SPECIAL EQUIPMENT,1) Balance,2) 2-2.0 L beakers and lids (watch glasses),3) Smooth magnetic stirring bar, teflon coated (1.5 cm),4) Stirrer hot plates,5) 2 L percolator, premarked at 250 and 1700 mL levels,ExFLP-21,- 2 - April 1997,Published on the Food Directorate’s (Health Canada's) website at http://www.hc-sc.gc.ca/food-aliment.,6) Rubber policeman attached to a long glass rod,7) Wash bottles,8) Suction filtration apparatus with Buchner or Hirsch funnel (5-7 cm perforated plate),9) Ruled filter paper. Filter paper should be larger than funnel plate (7-9 cm),10) Petri dishes to suit size of filter paper used,11) Stereoscopic microscope (10-30x),12) Ashless filter paper. Filter paper should be larger than funnel plate (7-9 cm),13) Drying oven (40-60oC),14) Crucible,15) Desiccator,16) Muffle oven,17) Concentrated HCl,18) Antifoam solution. Antifoam "B" (BDH Chemicals),19) Mineral oil. Paraffin oil, white, light 125/135 Saybolt Universal Viscosity (38oC), specific gravity,0.840-0.860 (24oC),20) Alcohol, either 95% ethanol or 99% isopropanol,21) Detergent, 1% sodium lauryl sulphate,22) Glycerol-alcohol (95%) mixture (1:1),23) Chloroform (CHCl3),5. PROCEDURE,The examination shall be carried out in accordance with the following instructions.,5.1 Preparation of Analytical Units,5.1.1 Thoroughly mix a sample unit and in a random fashion weigh 100 g. This 100 g constitutes an,analytical unit.,5.1.2 Repeat Step 5.1.1 for each of the remaining two sample units.,5.2 Isolation - Light Filth,5.2.1 Transfer an analytical unit to a 2 L beaker. Add 1 L HCl (50 mL concentrated HC1 + 950 mL water),0.3 mL of antifoam solution and a magnetic stirring bar.,ExFLP-21,- 3 - April 1997,5.2.2 Boil with intermittent stirring until mixture is finely dispersed (15-30 min.).,5.2.3 Transfer beaker to a cool magnetic stirrer, add 50 mL of mineral oil and stir magnetically at maximum,speed that does not produce visible or audible splashing for 5 min.,5.2.4 Promptly transfer contents of beaker to a percolator containing approximately 250 mL of water. Rinse,contents of beaker into percolator with hot tap water making certain to transfer all material including,heavy filth. Bring volume in percolator to 1700 mL with hot tap water. After 1 min., stir percolator,contents with a glass rod.,5.2.5 Using wash bottles, rinse magnetic particles from stirring bar into a beaker first with water, then alcohol,and finally with detergent until all particles are washed off. Filter washings through ruled filter paper,using suction filtration apparatus. Transfer filter paper to a petri dish and moisten with glycerol-alcohol,mixture.,5.2.6 After 2 min., drain oil interface in percolator to 250 mL into a 2 L beaker. Retain contents of beaker for,heavy filth determination.,5.2.7 Refill percolator by pouring hot tap water down percolator sides to loosen adhering material and refill to,1700 mL mark.,5.2.8 After 2-3 min., drain percolator to 250 mL, discard drainings and refill for 2 or more cycles. Lower layer,should be almost free of suspended m……
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